Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231)
Loading amount
100000 cells
Specification
MDA-MB-231
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 72-102 kDa
Positive control
MDA-MB-231 cells.
Additional data
Additional Notes
Cell lysate harvested with RIPA buffer + protease inhibitor tablet. Primary antibody tested at concentrations between 1/200-1/5000. Best results achieved at 1/500.
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提交于 May 04 2012