PerCP荧光/Cy5.5® Anti-CD14抗体[MEM-15],prediluted (ab157312)
Key features and details
- PerCP/Cy5.5® Mouse monoclonal [MEM-15] to CD14, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human, Non human primates
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG1
概述
-
产品名称
PerCP荧光/Cy5.5® Anti-CD14抗体[MEM-15],prediluted
参阅全部 CD14 一抗 -
描述
PerCP荧光/Cy5.5®小鼠单克隆抗体[MEM-15] to CD14,prediluted -
宿主
Mouse -
偶联物
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human, Non human primates -
免疫原
A crude mixture of Human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
-
阳性对照
- Human blood cells.
-
常规说明
Purified antibody is conjugated with tandem dye PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading...
-
纯度
Size exclusion -
克隆
单克隆 -
克隆编号
MEM-15 -
同种型
IgG1 -
研究领域
相关产品
-
Alternative Versions
- APC/Cy7® Anti-CD14 antibody [MEM-15], prediluted (ab194805)
- PE/Cy5® Anti-CD14 antibody [MEM-15] (ab232910)
- PE/Cy7® Anti-CD14 antibody [MEM-15] (ab234214)
- Alexa Fluor® 700 Anti-CD14 antibody [MEM-15] (ab269326)
- PerCP Anti-CD14 antibody [MEM-15] (ab269327)
- FITC Anti-CD14 antibody [MEM-15] (ab28061)
- Biotin Anti-CD14 antibody [MEM-15] (ab28118)
- APC Anti-CD14 antibody [MEM-15] (ab60901)
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab157312于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use 4µl for 106 cells.
4 μl reagent / 100 μl of whole blood. ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
---|
Flow Cyt
Use 4µl for 106 cells. 4 μl reagent / 100 μl of whole blood. ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
组织特异性
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
序列相似性
Contains 11 LRR (leucine-rich) repeats. -
翻译后修饰
N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
-
别名
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
see all
数据表及文件
-
SDS download
-
Datasheet download
文献 (4)
ab157312 被引用在 4 文献中.
- Kozbial A et al. Effect of Monocyte Seeding Density on Dendritic Cell Generation in an Automated Perfusion-Based Culture System. Biochem Eng J 150:N/A (2019). PubMed: 32831621
- Kozbial A et al. Automated generation of immature dendritic cells in a single-use system. J Immunol Methods 457:53-65 (2018). PubMed: 29625078
- Sing A et al. Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression. J Exp Med 196:1017-24 (2002). PubMed: 12391013
- Funda DP et al. CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited. Infect Immun 69:3772-81 (2001). PubMed: 11349042