Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH7
Permeabilization
Yes - Methanol/H2O2
Specification
Brain
Blocking step
A/B&DAKOprotein block & 2% bovine serum as blocking agent for 40 minute(s) · Concentration: 0% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: overnight, humidifying chamber, darkroom
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Biotinylated Anti-Rat IgG (H+L)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Citrisolv was used instead of xylene.
Methanol/H2O2 was made at 1:5
Citrate buffer was made at 1:100
Antibodies and AB solution (1:200) were diluted in TBS/FBS.
Washes were done in TBS/0.03%Tween20 for 30mins changing the wash solution every 10mins.
Image attached is a control mouse brain cerebellum, tumor area.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 06 2018