Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (synovium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid
Permeabilization
No
Specification
synovium
Blocking step
SuperBlock™ (PBS) Blocking Buffer as blocking agent for 15 minute(s) · Concentration: 0% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: BSA
Concentration
5 µg/ml
Secondary antibody
Name
Non-Abcam antibody was used: EnVision+
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
This antibody did not stain macrophage in rabbit sections.
Abcam response
Thank you for submitting this abreview. We are sorry to hear that you have problems with this antibody. This antibody has not been tested enough in IHC-P. We will welcome more data or suggestion from our cusomers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 28 2018