概述

  • 产品名称
    Anti-CD105抗体[MEM-226]
    参阅全部 CD105 一抗
  • 描述
    小鼠单克隆抗体[MEM-226] to CD105
  • 宿主
    Mouse
  • 特异性
    This antibody recognises CD105 antigen.
  • 经测试应用
    适用于: ICC/IF, Sandwich ELISA, Flow Cyt, IP, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant full length protein (Human). Expressed in vaccinia virus containing CD105 cDNA.

  • 阳性对照
    • ICC/IF: HeLa cells. WB: Human colon tissue lysate. FC: U937 cells.

性能

应用

Our Abpromise guarantee covers the use of ab2529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to CD105 (ab21224). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to CD105 (ab21224) as Detection.
Flow Cyt Use a concentration of 1 - 2 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition.

靶标

  • 功能
    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • 组织特异性
    Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • 疾病相关
    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI528660 antibody
    • AI662476 antibody
    • CD 105 antibody
    • CD105 antibody
    • CD105 antigen antibody
    • EGLN_HUMAN antibody
    • END antibody
    • Endoglin antibody
    • Eng antibody
    • FLJ41744 antibody
    • HHT1 antibody
    • ORW antibody
    • ORW1 antibody
    • Osler Rendu Weber syndrome 1 antibody
    • RP11 228B15.2 antibody
    • S-endoglin antibody
    • SN6 antibody
    see all

图片

  • Overlay histogram showing U937 (Human histiocytic lymphoma cell line) cells stained with ab2529 (red line).

    The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2529, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in U937 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ICC/IF image of ab2529 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

    The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2529, 10 µg/ml) overnight at +4°C. The secondary antibody (green) was ab69879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

  • Standard Curve for CD105 (Analyte: CD105 protein (ab54338)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-226] to CD105 (ab2529) at 5ug/ml and Detector Antibody Rabbit polyclonal to CD105 (ab21224) at 0.5ug/ml.
  • Anti-CD105 antibody [MEM-226] (ab2529) at 5 µg/ml + Human colon tissue lysate - total protein (ab30051) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size: 80 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 45 kDa, 55 kDa. We are unsure as to the identity of these extra bands.

文献

This product has been referenced in:
  • Shao B  et al. Regulatory effects of miRNA-181a on FasL expression in bone marrow mesenchymal stem cells and its effect on CD4+T lymphocyte apoptosis in estrogen deficiency-induced osteoporosis. Mol Med Rep 18:920-930 (2018). Read more (PubMed: 29845202) »
  • Rawal S  et al. Integration of mesenchymal stem cells into islet cell spheroids improves long-term viability, but not islet function. Islets 9:87-98 (2017). Read more (PubMed: 28662368) »
See all 11 Publications for this product

客户评价及客户问答

1-2 of 2 Abreviews or Q&A

Answer

I'm sorry to hear you are having problems with ab12529; this antibody has not been tested for FACS so it is very difficult to know if the problem can be solved with protocol problems or is due to the antibody not working well in FACS. As the antibody has been tested in IHC-P it means the antibody has the ability to recognise the native form of the protein, however this may require fixation and other protocol changes to get it working. The antibody has been tested in human brain tissue, so I would recommend trying this positive control if possible in IHC-P. The antibody has been purified on a peptide immunogen affinity column so it is purified and recognizes only GPCR EX33. You may be able to decrease the non specific staining with decreasing the antibody concentration and incubation conditions and changing the buffer. Could you please provide us your specific protocol details and we will look into this problem in more detail and hopefully will be able to suggest some tips? I enclose below a link to a protocol questionnaire to help you put your protocol information together easily, we look forward to hearing from you to help you more, https://www.abcam.com/index.html?section=facs&pageconfig=technical&intAbID=12529&mode=questionaire

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Answer

Thank you for your enquiry. Unfortunately, we currently do not have an antibody or antiserum against rat CD105. To see if another company may have what you are looking for, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249). If you have any more questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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