参阅全部 CCT6A 一抗
经测试应用适用于: WB, ICC/IF, IHC-Pmore details
预测可用于: Mouse, Rat
Synthetic peptide derived from an internal sequence of Human CCT6A.
- Human breast carcinoma tissue; HepG2 cell extracts This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2
存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 0.88% Sodium chloride, 50% Glycerol
Note: PBS (without Mg2+ and Ca2+)
Concentration information loading...
纯度Immunogen affinity purified
纯化说明ab110905 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab110905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 58 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
相关性CCT6A is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of CCT6A, encoding different isoforms, have been characterized.
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ICC/IF image of ab110905 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab110905 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
ab110905, at a 1/50 dilution, staining CCT6A in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry. The image on the right is from tissue treated with the synthesized peptide.
All lanes : Anti-CCT6A antibody (ab110905) at 1/500 dilution
Lane 1 : HepG2 cell extracts
Lane 2 : HepG2 cell extracts with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
ab110905 has not yet been referenced specifically in any publications.