Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (cervix)
Specification
cervix
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Jackson Lab goat anti rabbit Cy2
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy2 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy2 ®
Additional data
Additional Notes
cells were fixed in 4% PFA for 15 min
permeabilization for 15 min in 0.5% Triton X100 in PBS
permeabilization for 15 min in 0.5% Triton X100 in PBS
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Alexander Rapp
Verified customer
提交于 Feb 01 2007