Application
Western blot
Sample
Human Cell lysate - whole cell (CaCo 2 Cell line)
Loading amount
200 µg
Specification
CaCo 2 Cell line
Gel Running Conditions
Reduced Denaturing (15 % gel)
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 2.5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Amersham ECL+ Reagent
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 22 kDa Non-specific: 37, 55 kDa
Positive control
Actin antibody at 1:50 Dilution (other company)
Negative control
None
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Oct 27 2008