Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (293 cells)
Specification
293 cells
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Other product details
Dilution
1/100
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: anti-avadin
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/200
Additional data
Additional Notes
This antibody had a lot of unspecific binding, which was confirmed by protein knockdown. The protein of interest was biotinalyted.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 30 2012