Anti-Bag3抗体(ab47124)
Key features and details
- Rabbit polyclonal to Bag3
- Suitable for: ICC/IF, IP, IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Bag3抗体
参阅全部 Bag3 一抗 -
描述
兔多克隆抗体to Bag3 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IP, IHC-P, IHC-Fr, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
A recombinant protein fragment corresponding to the C terminal 196 amino acids of human BAG3.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading...
-
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47124于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
|
|
IP |
Use at an assay dependent concentration.
|
|
IHC-P |
1/1000 - 1/5000.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
WB | (1) |
1/1000 - 1/2000. Predicted molecular weight: 62 kDa.
|
说明 |
---|
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-P
1/1000 - 1/5000. |
IHC-Fr
Use at an assay dependent concentration. |
WB
1/1000 - 1/2000. Predicted molecular weight: 62 kDa. |
靶标
-
功能
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. -
疾病相关
Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material. -
序列相似性
Contains 1 BAG domain.
Contains 2 WW domains. - Information by UniProt
-
数据库链接
- Entrez Gene: 9531 Human
- Entrez Gene: 29810 Mouse
- Omim: 603883 Human
- SwissProt: O95817 Human
- SwissProt: Q9JLV1 Mouse
- Unigene: 523309 Human
- Unigene: 84073 Mouse
-
别名
- BAG 3 antibody
- BAG family molecular chaperone regulator 3 antibody
- BAG-3 antibody
see all
图片
-
All lanes : Anti-Bag3 antibody (ab47124) at 1/2000 dilution
Lane 1 : Thymus lysates, total protein (20ug/lane)
Lane 2 : Spleen lysates, total protein (20ug/lane)
Lane 3 : Skeletal(quadriceps) lysates, total protein (20ug/lane)
Lane 4 : Skeletal(diaphragm) lysates, total protein (20ug/lane)
Lane 5 : Smooth (heart) muscle lysates, total protein (20ug/lane)
Predicted band size: 62 kDa
Observed band size: 86 kDa why is the actual band size different from the predicted?
Tissue lysates, normalized for total protein (20 µg/lane), were from a 4 week old male mouse. BAG3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens. -
ICC/IF image of ab47124 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47124) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Frozen mouse muscle tissue section stained for BAG3 expression using ab47124 at 1/2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG3 localizes with Z-disk proteins."
-
Immunohistochemistry of formalin fixed paraffin embedded human pancreas tissue sections using ab47124 at 1/2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.
-
Anti-Bag3 antibody (ab47124) + Hela lysate at 0.5 mg/ml
Predicted band size: 62 kDa -
Immunocytochemistry/Immunofluorescence of A431 cells stained with Anti-Bag3 antibody (ab47124) green. Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
实验方案
数据表及文件
-
Datasheet download
文献 (22)
ab47124 被引用在 22 文献中.
- Piccolella M et al. Retinoic Acid Downregulates HSPB8 Gene Expression in Human Breast Cancer Cells MCF-7. Front Oncol 11:652085 (2021). PubMed: 34136389
- Lee S et al. Targeting HSF1 as a Therapeutic Strategy for Multiple Mechanisms of EGFR Inhibitor Resistance in EGFR Mutant Non-Small-Cell Lung Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 34203709
- Tanboon J et al. Inflammatory features in sporadic late-onset nemaline myopathy are independent from monoclonal gammopathy. Brain Pathol 31:e12962 (2021). PubMed: 34043258
- Lu SZ et al. Suppression of astrocytic autophagy by aB-crystallin contributes to a-synuclein inclusion formation. Transl Neurodegener 8:3 (2019). PubMed: 30675347
- Sun H et al. FAM111B, a direct target of p53, promotes the malignant process of lung adenocarcinoma. Onco Targets Ther 12:2829-2842 (2019). PubMed: 31114230