Application
Immunocytochemistry
Sample
Zebrafish Cultured Cells (ZEM2S (ATCC CRL-2147™))
Permeabilization
Yes - 0.3% Triton
Specification
ZEM2S (ATCC CRL-2147™)
Blocking step
Normal Serum Goat containing 22.52 mg/mL of glycine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 4°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: overnight
Dilution
1/250
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Cy3-labeled anti-rabbit secondary antibody from Ja
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3
Additional data
Additional Notes
4% Paraformaldehyde was used (Electron Microscopy Sciences, Hatfield, PA, USA).
Primary and secondary antibodies were diluted in incubation buffer
containing 1% bovine serumalbumin (Calbiochem, San Diego, CA, USA), 0.25% carrageenan lambda, and 0.3% Triton X-100.
Abcam response
Thank you for submitting this review and your valuable feedback. We are pleased that this antibody worked well for you in zebrafish, a species that has not been tested before. We welcome any further feedback and AbReviews from other researchers who have used this antibody in zebrafish which will help us assess how well the antibody works in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Ana Maria Castrucci
Verified customer
提交于 Aug 09 2017