概述

  • 产品名称
    B-Phycoerythrin偶联试剂盒
  • 产品概述

    B-Phycoerythrin Conjugation Kit ab102871 uses a simple and quick process to conjugate an antibody to B-Phycoerythrin. It can also be used to conjugate other proteins or peptides.


    To conjugate an antibody to B-Phycoerythrin using this kit:
    - add modifier to antibody and incubate for 3 hrs
    - add quencher and incubate for 30 mins
    The conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.


    Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to B-Phycoerythrin.


     


     

  • 说明

    Amount and volume of antibody for conjugation to B-Phycoerythrin.

     Kit size   Recommended 
    amount of antibody
    1 
    Maximum antibody 
    volume2
    180 µg   3 x 60 µg 3 x 60 µL
    60 µg   60 µg  60 µL
    600 µg   600 µg 600 µL

    1 Kit is designed to give a 1:1 molar ratio of antibody to PE after conjugation.

    2 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 5mg/ml or < 0.5 mg/ml should be diluted /concentrated.

    The selling size of this product has been changed – it is now based on the amount of antibody that can be conjugated with the kit, not the amount of PE mix provided. The quantity of reagents provided have not changed or the amount of antibody that can be conjugated.

     

    Buffer Requirements for Conjugation

    Buffer should be pH 6.5-8.5.

    Compatible buffer constituents 
    If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

    50mM / 0.6% Tris1 0.1%/1% BSA2 50% glycerol
    0.1% sodium azide PBS Potassium phosphate
    Sodium chloride HEPES Sucrose
    Sodium citrate EDTA Trehalose

    1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
    2 1% BSA gives lower quality conjugates, BSA can also interfere with the use of the conjugated antibody in tissue staining.

    Incompatible buffer constituents

    Thiomerosal Proclin Glycine
    Arginine Glutathione DTT

    If a constituent of the buffer containing your antibody or protein is not listed above, please check the FAQ or contact us.

    Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

  • 经测试应用
    适用于: Conjugationmore details

性能

应用

Our Abpromise guarantee covers the use of ab102871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Conjugation Use at an assay dependent dilution.

图片

  • This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.

实验方案

文献

This product has been referenced in:
  • Jung M  et al. Supplementation of dietary germanium biotite enhances induction of the immune responses by foot-and-mouth disease vaccine in cattle. BMC Vet Res 10:179 (2014). Read more (PubMed: 25255918) »
See 1 Publication for this product

客户评价及客户问答

Answer

Thank you for contacting us.

We currently do not have any publication information regarding its use in sheep BMSCs. I am sorry we can't offer any tested data. This antibody however was tested with sheep lysates and gave good staining so it is more likely that the antibody with detect sheep CD34 target. The use of ab81289 (https://www.abcam.com/CD34-antibody-EP373Y-ab81289.html) comes with Abcam Abpromise guarantee which states we will refund your money if the antibody fails to detect the CD34 target.

https://www.abcam.com/index.html?pageconfig=abpromise

I can provide further opinion about CD90 antibodies could you send me goat CD90 protein sequence?

We have kits that can be used for conjugation of antibodies. Please check the following products

ab102884; https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-3-x-100µg-FITC-ab102884.html
ab102883; https://www.abcam.com/EasyLink-FITC-Conjugation-Kit-1-x-1mg-FITC-ab102883.html

ab102870; https://www.abcam.com/EasyLink-B-Phycoerythrin-Conjugation-Kit-1-x-100µg-B-PE-ab102870.html
ab102871; https://www.abcam.com/EasyLink-B-Phycoerythrin-Conjugation-Kit-3-x-100µg-B-PE-ab102871.html

For full list of conjugation kits please click; https://www.abcam.com/index.html?pageconfig=resource&rid=13148

We do not have distributor in Portugal you can buy directly from us.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册