Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (15% acrilamide)
Sample
Mouse Cell lysate - whole cell (NmMG)
Specification
NmMG
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Diluent: TBS-T Milk 1%
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti Rb
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Bands
Specific: 0 kDa Non-specific: 29, 32 kDa
Exposure
10 minute(s) and 0 second(s)
Additional data
Additional Notes
Not posible to detect specific band in mouse samples.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 01 2013