Application
Western blot
Sample
Human Purified protein (purified protein (BIODESIGN))
Loading amount
0.5 µg
Specification
purified protein (BIODESIGN)
Gel Running Conditions
Reduced Denaturing
Blocking step
5%mill+1%BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Other product details
Dilution
1/2000
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: anti-goat IgG-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL(West Dura)
Exposure
10 second(s)
Bands
Specific: 25 kDa Non-specific: 65, 48, 40, 35, 30 kDa
Additional data
Additional Notes
Unlike ab7613, it seems like ab7614 dosen't recognize human apoAI. However, this antibody may detect apoAI (25kDa protein) in mouse liver and brain. Also note, this antibody seems to strongly react to ~40KDa protein (burned out spot)
Abcam response
Thank you for your feedback. I am sorry to hear that you are having problems with ab7614. ab7614 has not been tested on human samples and your results suggest that it does not work on this species but that it does detect the mouse ApoAI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 11 2008