Application
Flow Cytometry
Sample
Human Cell (Jurkat cells)
Permeabilization
No
Gating Strategy
no gate
Specification
Jurkat cells
Preparation
Cell harvesting/tissue preparation method: cell culture
Sample buffer: Binding buffer
Sample buffer: Binding buffer
Fixation
none
Other product details
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: binding buffer
Dilution
1/1100
Secondary antibody
Dilution
1/100
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed
Additional data
Additional Notes
1 (unfilled) = jurkat cells untreated
2 (grey) = jurkat cell treated with 5µM Campotothecin for 4 hours
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 06 2018