WB: Use at a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 76 kDa). The observed molecular weight corresponds to earlier findings in literature (Wigge et al, Mol Biol Cell. 1997 Oct;8(10):2003-15.; PMID: 9348539). A minor band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Neurons, certain endocrine cell types and spermatocytes.