We had good results using the ABCAM ammonia kit for the detection of NH3 in our cell lysates. However, we wanted to see the distribution of NH3 in the intact cells to determine the intercellular variation. We were able to adapt the kit to allow for the in situ detection of ammonia. We chose two cell lines one strongly + for NH3 and one -. The key was to use formalin fixed cells AND to digest in protease prior to use. Under these conditions, we got an excellent signal in the + cells and no signal in the - cells.
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提交于 Dec 27 2013