Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Treatment
Doxorubicin for 3 hours
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBST
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 680
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 680
Detection
Detection method
Fluorescence detection
Bands
Specific: 250 kDa
Exposure
5 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Andrew Cobb
Verified customer
提交于 Jul 11 2013