概述

  • 产品名称
    Anti-APOA1BP抗体
    参阅全部 APOA1BP 一抗
  • 描述
    兔多克隆抗体to APOA1BP
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human APOA1BP

  • 阳性对照
    • Extracts from HeLa cells. A549 cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 32 kDa.
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.

靶标

  • 相关性
    APOA1BP interacts with apolipoprotein A-I (apoA-I), the major apolipoprotein of high-density lipoproteins (HDLs). It is secreted into some bodily fluids, and its synthesis and secretion are stimulated in vitro by incubating cells with apoA-I.
  • 细胞定位
    Mitochondrion. Secreted. Note: In sperm, secretion gradually increases during capacitation
  • 数据库链接
  • 别名
    • AI BP antibody
    • AIBP antibody
    • ApoA I binding protein antibody
    • Apolipoprotein A I binding protein antibody
    • MGC119143 antibody
    • MGC119144 antibody
    • MGC119145 antibody
    • NAD(P)H hydrate epimerase antibody
    • NAD(P)HX epimerase antibody
    • YjeF N terminal domain containing protein 1 antibody
    • yjeF_N1 antibody
    • YJEFN1 antibody
    see all

图片

  • All lanes : Anti-APOA1BP antibody (ab75114) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from HeLa cells with immunizing peptide at 5 µg

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 32 kDa
    Observed band size: 29 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescence analysis of APOA1BP in A549 cells, using ab75114, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

文献

ab75114 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Multiple cell lines)
Gel Running Conditions
Reduced Denaturing
Loading amount
70 µg
Specification
Multiple cell lines
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

提交于 Jun 15 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册