Synthetic peptide derived from an internal sequence of human APOA1BP
Extracts from HeLa cells. A549 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 32 kDa.
1/500 - 1/1000.
APOA1BP interacts with apolipoprotein A-I (apoA-I), the major apolipoprotein of high-density lipoproteins (HDLs). It is secreted into some bodily fluids, and its synthesis and secretion are stimulated in vitro by incubating cells with apoA-I.
Mitochondrion. Secreted. Note: In sperm, secretion gradually increases during capacitation
AI BP antibody
ApoA I binding protein antibody
Western blot - Anti-APOA1BP antibody (ab75114)
All lanes :
Anti-APOA1BP antibody (ab75114) at 1/500 dilution
Lane 1 :
Extracts from HeLa cells
Lane 2 :
Extracts from HeLa cells with immunizing peptide at 5 µg
Lysates/proteins at 20 µg per lane.
Predicted band size:
Observed band size:
29 kDa (
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-APOA1BP antibody (ab75114)
Immunofluorescence analysis of APOA1BP in A549 cells, using ab75114, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
has not yet been referenced specifically in any publications.
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