存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度Immunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab84035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
组织特异性Expressed principally in liver. Weakly expressed in kidney.
序列相似性Contains 1 fibrinogen C-terminal domain.
- Information by UniProt
- ANG 5 antibody
- ANG-5 antibody
- ANG5 antibody
All lanes : Anti-ANGPTL3 antibody (ab84035) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa (possible cleavage fragment)
Exposure time: 20 minutes
ANGPTL3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. We hypothesize that the 58 and 56 kDa bands represent the precursor and mature proteins, respectively.
ab84035 has not yet been referenced specifically in any publications.