The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 58 & 56 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Expressed principally in liver. Weakly expressed in kidney.
ANGPTL3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. We hypothesize that the 58 and 56 kDa bands represent the precursor and mature proteins, respectively.