Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain slide)
Specification
Brain slide
Other product details
Incubation time
72 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Biotin-SP (long spacer) AffiniPure F(ab')2 Fragmen
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Other information :
Rhodamine (TRITC) Streptavidin (Jackson 016 020 084) : 1/200 during 90 minutes at room temperature.
First antibody is diluted in a blocking buffer (2% donkey serum + 0.3% Triton X-100 + 0.01% NaN3 in TBS)
Secondary antibody is diluted in an incubation solution (0.3% Triton X-100 + 0.01% NaN3 in TBS) during 90 minutes at room temperature.
We observe a labelling of only glial cells (confirmed by co-staining with GFAP) while the expression of AMPK a1 should be mainly in neurons.
We have tried also the labelling after antigen retrieval but the results are the same.
Abcam response
Thank you for participating in our AbTrial testing program. We welcome additional feedback on this combination of application/species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Delphine Franssen
Verified customer
提交于 May 17 2017