Application
Western blot
Sample
Human Cell lysate - whole cell (human HepG2 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
human HepG2 cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS tween 0,05 %
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Amersham ECL Rabbit IgG, HRP-linked whole Ab (from
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 63 kDa
Additional data
Additional Notes
4 independent extractions from the human HepG2 cells extracted with a RIPA buffer
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提交于 Dec 05 2016