Anti-ALX3抗体(ab64985)
Key features and details
- Rabbit polyclonal to ALX3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ALX3抗体 -
描述
兔多克隆抗体to ALX3 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human ALX3 aa 150-250 (internal sequence).
Database link: O95076 -
阳性对照
- 293 cell extract and HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64985于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
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ICC/IF |
1/500 - 1/1000.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
ICC/IF
1/500 - 1/1000. |
靶标
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相关性
ALX3 (aristaless-like homeobox 3) is a nuclear protein with a homeobox DNA-binding domain. It functions as a transcriptional regulator with a possible role in patterning of the mesoderm during development. Preferential methylation of the ALX3 promoter is associated with advanced-stage neuroblastoma tumors. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 257 Human
- Entrez Gene: 11694 Mouse
- Entrez Gene: 365900 Rat
- Omim: 606014 Human
- SwissProt: O95076 Human
- SwissProt: O70137 Mouse
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别名
- ALX 3 antibody
- ALX3 homeobox 3 antibody
- Aristaless like homeobox 3 antibody
see all
图片
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All lanes : Anti-ALX3 antibody (ab64985) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : 293 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence analysis of HeLa cells, using ab64985 at 1/500-1/1000 dilution. Right hand panel was also treated with immunising peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab64985 被引用在 4 文献中.
- Qi J et al. Oncogenic role of ALX3 in cervical cancer cells through KDM2B-mediated histone demethylation of CDC25A. BMC Cancer 21:819 (2021). PubMed: 34266408
- He L et al. Parenchymal and stromal tissue regeneration of tooth organ by pivotal signals reinstated in decellularized matrix. Nat Mater 18:627-637 (2019). PubMed: 31114073
- García-Sanz P et al. Embryonic defence mechanisms against glucose-dependent oxidative stress require enhanced expression of Alx3 to prevent malformations during diabetic pregnancy. Sci Rep 7:389 (2017). PubMed: 28341857
- Mirasierra M & Vallejo M Glucose-dependent downregulation of glucagon gene expression mediated by selective interactions between ALX3 and PAX6 in mouse alpha cells. Diabetologia 59:766-75 (2016). PubMed: 26739814