Immunocytochemistry abreview for Anti-AKT3 + AKT2 + AKT1 antibody [Y89]

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.03%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1x TBS with 0.1% Blocking
Dilution
1/100

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

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提交于 Feb 08 2018

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