Western blot abreview for Anti-AKT1 antibody [Y89]

Excellent
Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Incubation time
13 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1xTBS
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
Chemiluminescence
Bands
Specific: 60 kDa Non-specific: none kDa
Exposure
30 second(s)

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提交于 Feb 11 2015

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