Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal Muscle)
Gel Running Conditions
Reduced Denaturing (4%-12%)
Loading amount
10 µg
Specification
Skeletal Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST with 2.5%BSA
Dilution
1/3000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Polyclonal Goat Anti Rabbit Immunoglobulins/HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 110 kDa
Positive control
Actn3 is highly expressed in skeletal muscle. Tibialis anterior muscle tissue lysate was used for western blot.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 25 2016