Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (Fibroblasts)
Specification
Fibroblasts
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyL
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Dyomics 647
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Dyomics 647
Dilution
1/5000
Detection
Detection method
fluorescence
Bands
Specific: 80 kDa
Exposure
2 minute(s) and 0 second(s)
Additional data
Additional Notes
Samples are human fibroblats
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 14 2013