A431核extract裂解物(EGF treated) (ab14635)

概述

  • 产品名称
    A431核extract裂解物(EGF treated)
    参阅全部 A431 细胞裂解液
  • 描述
    A431核extract裂解物
  • 常规说明
    Cell line: A431 (Human epithelial carcinoma).
    Cells were cultured in medium supplemented with 10 ng/mL EGF (epidermal growth factor) for 10 minutes at 37°C immediately prior to harvesting.
    We recommend aliquoting the extracts into single use fractions and then storing.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT, 10 ng/mL EGF (epidermal growth factor). pH 7.9
  • Concentration information loading...
  • 研究领域
  • 背景
    A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.

文献

ab14635 has not yet been referenced specifically in any publications.

客户评价及客户问答

1-2 of 2 Q&A

Answer

J'ai mis en place l'envoi d'une unité gratuite de lysat de A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated, référence ab14635. Le numéro de commande est : *****.

Ce lysat est recommandé comme contrôle positif pour STAT5 phosphorylé et devrait convenir également pour l'anti-STAT5 ab7969.

J'espère que ce lysat vous sera utile. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Question
Answer

Thank you for your enquiry. ab14635 (A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated) has been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). Positive Transcription Factors in Western: c-Jun, C/EBPb, Sp1, YY1 and in EMSA: Sp1, USF, YY1. Please don't hesitate to contact us again if you have further questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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