8-hydroxy 2 deoxyguanosine ELISA试剂盒(ab201734)
Key features and details
- Sensitivity: 0.59 ng/ml
- Range: 0.94 ng/ml - 60 ng/ml
- Sample type: Cell Lysate, Plasma, Saliva, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Competitive
概述
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产品名称
8-hydroxy 2 deoxyguanosine ELISA试剂盒
参阅全部 8-hydroxy 2 deoxyguanosine 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Spiked 90 < 5% 批次间 样品 n Mean SD CV% Spiked 90 < 5% -
样品类型
Saliva, Urine, Serum, Plasma, Tissue Extracts, Cell Lysate -
检测类型
Competitive -
灵敏度
= 0.59 ng/ml -
范围
0.94 ng/ml - 60 ng/ml -
检测时间
2h 00m -
实验步骤
Multiple steps standard assay -
产品概述
8-hydroxy 2 deoxyguanosine ELISA Kit (8-OHdG) (ab201734) is a competitive assay that can be used for the quantification of 8-OHdG in urine, cell culture, plasma, and other sample matrices. The ELISA utilizes an 8-hydroxy-2-deoxyguanosine-coated plate and an HRP-conjugated antibody for detection which allows for an assay range of 0.94 - 60 ng/mL, with a sensitivity of 0.59 ng/mL. The other highlights of this kit are a quick incubation time of 60 minutes, stable reagents, and an easy to use protocol.
It is important to note that the 8-OHdG antibody used in this assay recognizes both free 8-OHdG and DNA-incorporated 8-OHdG. Since complex samples such as plasma, cell lysates, and tissues are comprised of mixtures of DNA fragments and free 8-OHdG, concentrations of 8-OHdG reported by ELISA methodology will not coincide with those reported by LC-MS where the single nucleoside is typically measured. This should be kept in mind when analyzing and interpreting experimental results.
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说明
8-hydroxy-2-deoxy Guanosine (8-OHdG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress. Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OHdG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension.
In complex samples such as plasma, cell lysates, and tissues, 8-OHdG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OHdG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OHdG than plasma. Urinary levels of 8-OHdG range between 2.7- 13 ng/mg creatine, while plasma levels of free 8-OHdG have been reported to be between 4-21 pg/mL as determined by LC-MS.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 8-hydroxy-2-deoxy Guanosine Antibody Diluent (Blue) 1 x 13ml 8-Hydroxy-2-Deoxy Guanosine BSA Coated Plate 1 unit 8-hydroxy-2-deoxy Guanosine HRP Conjugated Monoclonal Antibody 1 x 75µl 8-hydroxy-2-deoxy Guanosine Sample and Standard Diluent (Red) 1 x 50ml 8-hydroxy-2-deoxy Guanosine Standard 1 x 100µl 8-hydroxy-2-deoxy Guanosine Stop Solution 1 x 13ml 8-hydroxy-2-deoxy Guanosine TMB Substrate 1 x 13ml 8-hydroxy-2-deoxy Guanosine Wash Buffer Concentrate (10X) 1 x 50ml Plate Cover 2 units -
研究领域
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相关性
Oxidative damage of DNA or RNA by reactive oxygen and nitrogen species produces 8-hydroxy Guanine. This can serve as a measure of oxidative stress. -
别名
- 8 OH dG
图片
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8-hydroxy 2 deoxyguanosine measured in urine (dilution test range: 1/10-1/200; duplicates; +/- SD).
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Standard curve with background signal subtracted (duplicates; +/- SD).
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Abcam's 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734) Typical standard curve
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Abcam's 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734) Schematic
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Abcam's 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734) Recovery of 8-hydroxy-2-deoxy Guanosine from urine
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Abcam's 8-hydroxy 2 deoxyguanosine ELISA Kit (ab201734) Oxidation of Guanosine
数据表及文件
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SDS download
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Datasheet download
文献 (82)
ab201734 被引用在 82 文献中.
- McGlone CL et al. Evidence for Systemic Reactive Oxygen Species in UVB-mediated Microvesicle Formation. Photochem Photobiol 98:242-247 (2022). PubMed: 34324712
- Guo C et al. MicroRNA-214-5p aggravates sepsis-related acute kidney injury in mice. Drug Dev Res 83:339-350 (2022). PubMed: 34370322
- Yao X et al. In-cytoplasm mitochondrial transplantation for mesenchymal stem cells engineering and tissue regeneration. Bioeng Transl Med 7:e10250 (2022). PubMed: 35111950
- Wu J et al. TNF antagonist sensitizes synovial fibroblasts to ferroptotic cell death in collagen-induced arthritis mouse models. Nat Commun 13:676 (2022). PubMed: 35115492
- Duan W et al. Cervical Vagus Nerve Stimulation Improves Neurologic Outcome After Cardiac Arrest in Mice by Attenuating Oxidative Stress and Excessive Autophagy. Neuromodulation 25:414-423 (2022). PubMed: 35131154