重组Anti-6X His tag®抗体[RMH01] (ab219465)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [RMH01] to 6X His tag®
- Suitable for: ELISA, Sandwich ELISA, WB
- Reacts with: Species independent
概述
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产品名称
Anti-6X His tag®抗体[RMH01]
参阅全部 6X His tag® 一抗 -
描述
人单克隆抗体[RMH01] to 6X His tag® -
宿主
Human -
特异性
ab219465 reacts to recombinant proteins containing the 6X His tag® or 10X His tag® fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
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经测试应用
适用于: ELISA, Sandwich ELISA, WBmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide (His-tag). (Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus).
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阳性对照
- WB: 293T cells transfected with a DNA construct encoding His-Tag Histone H3 (K36M) protein; Sandwich ELISA: 293T cells transfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein; purified HisTag-PCSK9 recombinant protein
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常规说明
ab219465 is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (ab200537), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
ab219465 was purified from an animal origin–free culture supernatant. -
克隆
单克隆 -
克隆编号
RMH01 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab219465于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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Sandwich ELISA |
Use at an assay dependent concentration.
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|
WB |
Use a concentration of 0.2 µg/ml.
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说明 |
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ELISA
Use at an assay dependent concentration. |
Sandwich ELISA
Use at an assay dependent concentration. |
WB
Use a concentration of 0.2 µg/ml. |
靶标
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相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
图片
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Sandwich ELISA performed on different amounts of whole lysate of 293T cells transfected with a DNA construct encoding HisTag Histone H3 (K36M) protein, using 50µL/well of different concentrations of ab219465 as the capture antibody. Anti-H3K36M rabbit monoclonal antibody (RM193) was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-Rabbit IgG as the Secondary antibody.
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A titer ELISA performed on purified HisTag-PCSK9 recombinant protein. A serial dilution of ab219465 was used as the primary antibody. An alkaline phosphatase conjugated anti-Human IgG as the secondary antibody
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All lanes : Anti-6X His tag® antibody [RMH01] (ab219465) at 0.2 µg/ml
Lane 1 : 293T cells transfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein
Lane 2 : 293T cells untransfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein
Secondary
All lanes : HRP conjugated anti-Human IgG secondary antibody
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab219465 被引用在 1 文献中.
- Luo S et al. Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion. Oncol Lett 15:7760-7768 (2018). PubMed: 29725470