Application
Western blot
Sample
Escherichia coli Purified protein (BL21(DE3))
Loading amount
0.01 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/4000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBST+5%Milk
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: 44 kDa
Additional data
Additional Notes
Loaded 10ng & 30ng same sample onto the gel.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 30 2008