产品名称Anti-6X His tag®抗体(HRP)
参阅全部 6X His tag® 一抗
描述兔多克隆抗体to 6X His tag® (HRP)
经测试应用适用于: ELISA, WBmore details
种属反应性与反应: Species independent
Synthetic peptide: HHHHHH conjugated to KLH.
- E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) can be used as a positive control in WB.
存放说明Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
- Anti-6X His tag® antibody [4D11] (HRP) (ab202004)
Our Abpromise guarantee covers the use of ab1187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/5000 - 1/20000.|
|WB||1/1000 - 1/5000.|
相关性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
细胞定位Depends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
All lanes : Anti-6X His tag® antibody (HRP) (ab1187) at 1/4000 dilution
Lane 1 : 4ug Human His-tagged protein purified from E.coli
Lane 2 : 2ug Human His-tagged protein purified from E.coli
Lane 3 : 1ug Human His-tagged protein purified from E.coli
Lane 4 : 0.5ug Human His-tagged protein purified from E.coli
Observed band size: 136 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Li S et al. The AREB1 Transcription Factor Influences Histone Acetylation to Regulate Drought Responses and Tolerance in Populus trichocarpa. Plant Cell 31:663-686 (2019). Read more (PubMed: 30538157) »
- Golinski AW et al. Biophysical Characterization Platform Informs Protein Scaffold Evolvability. ACS Comb Sci 21:323-335 (2019). Read more (PubMed: 30681831) »