概述

  • 产品名称

    Anti-6X His tag®抗体(HRP)
    参阅全部 6X His tag® 一抗
  • 描述

    兔多克隆抗体to 6X His tag® (HRP)
  • 宿主

    Rabbit
  • 偶联物

    HRP
  • 经测试应用

    适用于: ELISA, WBmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Synthetic peptide: HHHHHH conjugated to KLH.

  • 阳性对照

    • E. coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 6X His tag protein (ab2431) can be used as a positive control in WB.
  • 常规说明

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000 - 1/20000.
WB 1/1000 - 1/5000.

靶标

  • 相关性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

图片

  • All lanes : Anti-6X His tag® antibody (HRP) (ab1187) at 1/4000 dilution

    Lane 1 : 4ug Human His-tagged protein purified from E.coli
    Lane 2 : 2ug Human His-tagged protein purified from E.coli
    Lane 3 : 1ug Human His-tagged protein purified from E.coli
    Lane 4 : 0.5ug Human His-tagged protein purified from E.coli

    Observed band size: 136 kDa
    why is the actual band size different from the predicted?

    See Abreview

实验方案

文献

This product has been referenced in:

  • Li S  et al. The AREB1 Transcription Factor Influences Histone Acetylation to Regulate Drought Responses and Tolerance in Populus trichocarpa. Plant Cell 31:663-686 (2019). Read more (PubMed: 30538157) »
  • Golinski AW  et al. Biophysical Characterization Platform Informs Protein Scaffold Evolvability. ACS Comb Sci 21:323-335 (2019). Read more (PubMed: 30681831) »
See all 70 Publications for this product

客户评价及客户问答

1-10 of 16 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse pancreatic cancer primary cell lines)
Loading amount
25 µg
Specification
mouse pancreatic cancer primary cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Feb 09 2011

Western blot

Excellent
Abreviews
Application
Western blot
Loading amount
0.1 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Recombinant protein (purification sur colonne HIS Trap)
Specification
purification sur colonne HIS Trap
Blocking step
caséine as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C

Abcam user community

Verified customer

提交于 May 30 2013

Question
Answer

 Thank you for calling Abcam earlier. After having spoken to the lab, I would advise you not to store ab1187 at at -20C as it will cause the product to lose some functionality due to the HRP conjugation.

Read More
Application
Western blot
Sample
Human Purified protein (purified His-tagged lipase, SPINK1)
Loading amount
1 µg
Specification
purified His-tagged lipase, SPINK1
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Miklos Sahin-Toth

Verified customer

提交于 Aug 12 2010

Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuronal cell)
Loading amount
20 µg
Specification
neuronal cell
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

提交于 Feb 09 2010

Application
Western blot
Sample
Human Purified protein (Purified His-tagged extracellular human LDLR)
Loading amount
0.1 µg
Specification
Purified His-tagged extracellular human LDLR
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

提交于 Aug 25 2009

Application
ELISA
Sample
Escherichia coli Purified protein (BL21(DE3))
Specification
BL21(DE3)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Type
Sandwich (Capture)

Abcam user community

Verified customer

提交于 Jan 27 2009

Application
Western blot
Sample
Escherichia coli Purified protein (BL21(DE3))
Loading amount
0.01 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Dec 30 2008

Application
Western blot
Sample
Human Purified protein (recombinant human fibronectin domain expressed in)
Loading amount
1 µg
Specification
recombinant human fibronectin domain expressed in
Gel Running Conditions
Reduced Denaturing (12% BisTris NuPAGE gel)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

提交于 Sep 09 2008

Application
ELISA
Sample
Rat Serum (injected his-yeagged peptide)
Specification
injected his-yeagged peptide
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Type
Sandwich (Detection)

Dr. Sandra Reichstetter

Verified customer

提交于 Dec 11 2007

1-10 of 16 Abreviews or Q&A

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