Application
Western blot
Sample
Fish Recombinant protein (sf9)
Gel Running Conditions
Reduced Non-Denaturing (Native) (10% SDS)
Loading amount
2e+007 cells
Specification
sf9
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Goat anti-mouse IgG1-AP
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
NBT/BCIP
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 36.1 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 02 2020