Application
Western blot
Loading amount
1 µg
Gel Running Conditions
Reduced Denaturing (12% hand cast gel)
Sample
Escherichia coli Recombinant protein (6x His-tagged protein expressed in BL21 (pET15b))
Specification
6x His-tagged protein expressed in BL21 (pET15b)
Blocking step
Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: OBB (TBS) + 0.2% Tween20
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Dilution
1/5000
Detection
Detection method
LI-COR infrared imaging
Bands
Specific: 30 kDa
Exposure
5 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Rachel Loh
Verified customer
提交于 Jan 15 2015