Anti-6X His tag®抗体[HIS.H8] (ab18184)
概述
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产品名称
Anti-6X His tag®抗体[HIS.H8]
参阅全部 6X His tag® 一抗 -
描述
小鼠单克隆抗体[HIS.H8] to 6X His tag® -
宿主
Mouse -
特异性
Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells. -
经测试应用
适用于: ICC/IF, ELISA, Dot blot, WB, IP, EMSAmore details -
种属反应性
与反应: Species independent -
免疫原
6x His synthetic peptide.
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阳性对照
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常规说明
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from TCS by Protein A affinity chromatography -
克隆
单克隆 -
克隆编号
HIS.H8 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab18184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/200. | |
| ELISA | Use at an assay dependent dilution. | |
| Dot blot | Use at an assay dependent dilution. | |
| WB | 1/1000. | |
| IP | 1/200. | |
| EMSA | Use at an assay dependent dilution. PubMed: 20606007 |
靶标
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相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
图片
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![Immunoprecipitation - Anti-6X His tag® antibody [HIS.H8] (ab18184)](https://www.abcam.cn/ps/products/18/ab18184/Images/ab18184-26832-anti-6x-his-tag-antibody-hish8-immunoprecipitation.jpg)
Immunoprecipitation - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an Abreview submitted by Mr Chris TracyImmunoprecipitation of His/FLAG tagged PDCD5 using ab18184 at 1/100 dilution.
Lane 1 : MW Ladder (from top 120, 85, 50, 34, 25, 19 kDa)
Lanes 2-5 : Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 from mixture of ~50 ng purified His/FLAG tagged PDCD5 and 15 µL RRL in 300 µL buffer.
Lanes 6-7 : Purified PDCD5 from E. coli with ~60-70 ng protein.
The lowest band in each lane is ~ 20 kDa and is PDCD5. The upper bands in lanes 2-5 are the heavy chain (~50 kDa, runs as doublet) and light chain (~25 or 26 kDa).
Western Blot was performed with a mouse anti-FLAG primary antibody at 1/2000 dilution. An IRDye®800CW-conjugated goat anti-mouse IgG was used as a secondary antibody. -
![Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)](https://www.abcam.cn/ps/products/18/ab18184/Images/ab18184-261409-anti-6x-his-tag-antibody-hish8-western-blot.jpg)
Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an Abreview submitted by Vladimir MilenkovicAll lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/5000 dilution
Lane 1 : ARPE-19 whole cell lysate - non-transfected
Lane 2 : ARPE-19 whole cell lysate - transfected with rRab27a-His
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-mouse IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 5 minutesBlocked with 5% milk for 30 minutes at 25°C.
Incubated with the primary antibody for 16 hours at 4°C in 1X PBS/Tween, 5% milk.
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![Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)](https://www.abcam.cn/ps/products/18/ab18184/Images/ab18184-290288-anti-6x-his-tag-antibody-hish8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).
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![Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)](https://www.abcam.cn/ps/products/18/ab18184/Images/ab18184-261408-anti-6x-his-tag-antibody-hish8-western-blot.jpg)
Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an Abreview submitted by Rachel LohAll lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution
Lane 1 : 6x His-tagged protein expressed in BL21 (pET15b) at 1 µg
Lane 2 : 6x His-tagged protein expressed in BL21 (pET15b) at 0.1 µg
Secondary
All lanes : IRDye® 800CW goat anti-mouse IgG (H+L) polyclonal at 1/5000 dilution
Performed under reducing conditions.
Exposure time: 5 minutes
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![Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)](https://www.abcam.cn/ps/products/18/ab18184/Images/ab18184-26833-anti-6x-his-tag-antibody-hish8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an anonymous abreview.ab18184 at a 1/1000 dilution staining 6X His tag in transfected human HEK293 cells by Immunocytochemistry/ Immunofluorescence incubated for 12 hours at 22°C. PFA fixed. Permeabilized using 0.1% Triton X-100 in PBS. Blocked using 3% BSA for 1 hour at 22°C. Secondary used at a 1/1000 dilution polyclonal Goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 (green). DAPI staining (blue).
实验方案
文献
ab18184 被引用在 152 文献中.
- Li Y et al. Characteristics of a Novel Manganese Superoxide Dismutase of a Hadal Sea Cucumber (Paelopatides sp.) from the Mariana Trench. Mar Drugs 17:N/A (2019). PubMed: 30717090
- Gu Y et al. Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01. Nat Commun 10:893 (2019). PubMed: 30792391
- Debus JD et al. In vitro analysis of arrhythmogenic cardiomyopathy associated desmoglein-2 (DSG2) mutations reveals diverse glycosylation patterns. J Mol Cell Cardiol 129:303-313 (2019). PubMed: 30885746
- Paydarnia N et al. Synergistic effect of granzyme B-azurin fusion protein on breast cancer cells. Mol Biol Rep N/A:N/A (2019). PubMed: 30937652
- Viplav A et al. ArhGEF37 assists dynamin 2 during clathrin-mediated endocytosis. J Cell Sci 132:N/A (2019). PubMed: 30926623
