Anti-6X His tag®抗体[HIS.H8] (ab18184)
![Immunoprecipitation - Anti-6X His tag® antibody [HIS.H8] (ab18184)](https://www.abcam.cn/ps/products/18/ab18184/Images/ab18184-26832-anti-6x-his-tag-antibody-hish8-immunoprecipitation.jpg "Immunoprecipitation - Anti-6X His tag® antibody [HIS.H8] (ab18184)")
Key features and details
- Mouse monoclonal [HIS.H8] to 6X His tag®
- Suitable for: ICC/IF, ELISA, Dot blot, WB, IP
- Reacts with: Species independent
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
Anti-6X His tag®抗体[HIS.H8]
参阅全部 6X His tag® 一抗 -
描述
小鼠单克隆抗体[HIS.H8] to 6X His tag® -
宿主
Mouse -
特异性
Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.
ab18184 reacts to recombinant proteins containing the 6X His tag® or 10X His tag® fused to either the amino or carboxy terminus.
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经测试应用
适用于: ICC/IF, ELISA, Dot blot, WB, IPmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to 6X His tag®.
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阳性对照
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常规说明
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from TCS by Protein A affinity chromatography -
克隆
单克隆 -
克隆编号
HIS.H8 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab18184于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (3) |
1/200.
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| ELISA | (1) |
Use at an assay dependent concentration.
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| Dot blot |
Use at an assay dependent concentration.
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| WB | (21) |
1/1000.
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| IP | (5) |
1/200.
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| 说明 |
|---|
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ICC/IF
1/200. |
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ELISA
Use at an assay dependent concentration. |
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Dot blot
Use at an assay dependent concentration. |
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WB
1/1000. |
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IP
1/200. |
靶标
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相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
图片
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Immunoprecipitation - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an Abreview submitted by Mr Chris Tracy
Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 at 1/100 dilution.
Lane 1 : MW Ladder (from top 120, 85, 50, 34, 25, 19 kDa)
Lanes 2-5 : Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 from mixture of ~50 ng purified His/FLAG tagged PDCD5 and 15 µL RRL in 300 µL buffer.
Lanes 6-9 : Purified PDCD5 from E. coli with ~60-70 ng protein.
The lowest band in each lane is ~ 20 kDa and is PDCD5. The upper bands in lanes 2-5 are the heavy chain (~50 kDa, runs as doublet) and light chain (~25 or 26 kDa).
Western Blot was performed with a mouse anti-FLAG primary antibody at 1/2000 dilution. An IRDye®800CW-conjugated goat anti-mouse IgG was used as a secondary antibody. -
![Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)](https://www.abcam.cn/ps/products/18/ab18184/Images/ab18184-261409-anti-6x-his-tag-antibody-hish8-western-blot.jpg)
Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an Abreview submitted by Vladimir MilenkovicAll lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/5000 dilution
Lane 1 : ARPE-19 whole cell lysate - non-transfected
Lane 2 : ARPE-19 whole cell lysate - transfected with rRab27a-His
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-mouse IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 5 minutesBlocked with 5% milk for 30 minutes at 25°C.
Incubated with the primary antibody for 16 hours at 4°C in 1X PBS/Tween, 5% milk.
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![Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)](https://www.abcam.cn/ps/products/18/ab18184/Images/ab18184-290288-anti-6x-his-tag-antibody-hish8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).
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![Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)](https://www.abcam.cn/ps/products/18/ab18184/Images/ab18184-261408-anti-6x-his-tag-antibody-hish8-western-blot.jpg)
Western blot - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an Abreview submitted by Rachel LohAll lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution
Lane 1 : 6x His-tagged protein expressed in BL21 (pET15b) at 1 µg
Lane 2 : 6x His-tagged protein expressed in BL21 (pET15b) at 0.1 µg
Secondary
All lanes : IRDye® 800CW goat anti-mouse IgG (H+L) polyclonal at 1/5000 dilution
Performed under reducing conditions.
Exposure time: 5 minutes
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![Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)](https://www.abcam.cn/ps/products/18/ab18184/Images/ab18184-26833-anti-6x-his-tag-antibody-hish8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [HIS.H8] (ab18184)This image is courtesy of an anonymous abreview.ab18184 at a 1/1000 dilution staining 6X His tag in transfected human HEK293 cells by Immunocytochemistry/ Immunofluorescence incubated for 12 hours at 22°C. PFA fixed. Permeabilized using 0.1% Triton X-100 in PBS. Blocked using 3% BSA for 1 hour at 22°C. Secondary used at a 1/1000 dilution polyclonal Goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 (green). DAPI staining (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (250)
ab18184 被引用在 250 文献中.
- Smith EM et al. The mTOR regulated RNA-binding protein LARP1 requires PABPC1 for guided mRNA interaction. Nucleic Acids Res 49:458-478 (2021). PubMed: 33332560
- Lim Y et al. Phosphorylation by Aurora B kinase regulates caspase-2 activity and function. Cell Death Differ 28:349-366 (2021). PubMed: 32811973
- Wang S et al. AXL is a candidate receptor for SARS-CoV-2 that promotes infection of pulmonary and bronchial epithelial cells. Cell Res 31:126-140 (2021). PubMed: 33420426
- Kwak MS et al. Reactive oxygen species induce Cys106-mediated anti-parallel HMGB1 dimerization that protects against DNA damage. Redox Biol 40:101858 (2021). PubMed: 33461096
- Calero-Cuenca FJ et al. Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration. Curr Biol 31:1521-1530.e8 (2021). PubMed: 33567288