概述

  • 产品名称

    Anti-6X His tag®抗体(FITC)
    参阅全部 6X His tag® 一抗
  • 描述

    山羊多克隆抗体to 6X His tag® (FITC)
  • 宿主

    Goat
  • 偶联物

    FITC. Ex: 493nm, Em: 528nm
  • 经测试应用

    适用于: ICCmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Synthetic peptide:

    HHHHHH

    (6-His) conjugated to KLH.

  • 常规说明

    HIS-TAG® is a trademark of EMD Biosciences, Inc.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituent: 0.2% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab1270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/200 - 1/2000.

靶标

  • 相关性

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • 细胞定位

    Depends upon the localization of the parent protein tagged with hexahistidine.
  • 别名

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

文献

This product has been referenced in:

  • Tai-Nagara I  et al. Placental labyrinth formation in mice requires endothelial FLRT2/UNC5B signaling. Development 144:2392-2401 (2017). Read more (PubMed: 28576770) »
See 1 Publication for this product

客户评价及客户问答

1-2 of 2 Abreviews or Q&A

Answer

The antibody is designed to react with 6 His at the N-terminus and C-terminus.

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Answer

The method of fixation is unlikely to have significant effect on the His-tag. However, the fixation may influence the conformation of the tagged protein, and thereby, affect the accessibility of the His-tag. Thus, one must empirically determine the influence of fixation upon ability to detect tagged proteins.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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