Application
Flow Cytometry
Sample
Human Cell (endometrial carcinoma)
Specification
endometrial carcinoma
Preparation
Cell harvesting/tissue preparation method: short trypsinisation
Sample buffer: PBS+1%FBS+0.02%NaN3
Sample buffer: PBS+1%FBS+0.02%NaN3
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
gate included all single cells
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: none
Secondary antibody
Name
Non-Abcam antibody was used: 6X His tag® antibody (FITC) (ab1206)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Additional Notes
In the experiment, a primary antibody carrying a 6xHis tag was used. So ab1206 was used as a secondary. On the image, red line represents cells stained with ab1206 alone, and the blue line is the tested sample carrying a 6xHis tag. The small peak to the right represents cells that bound the tested sample.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 07 2009