存放说明Shipped at 4°C. Store at +4°C.
存储溶液Constituents: PBS, 0.2% BSA
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纯度Immunogen affinity purified
纯化说明Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
Our Abpromise guarantee covers the use of ab1206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. PubMed: 21183632|
|Flow Cyt||Use at an assay dependent concentration.
ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
相关性The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
细胞定位Depends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
ab1206 staining 6X His tag® in Human endometrial carcinoma cells by Flow Cytometry. Cells were prepared by short trypsinisation in PBS/1% FBS/0.2% sodium azide and fixed with formaldehyde. The gate included all single cells. The sample was incubated with a 6X His tag® primary antibody for 1 hour at 4°C. Ab1206 was used as the secondary. On the image, the red line represents cells stained with ab1206 alone, and the blue line is the tested sample carrying a 6XHis tag®. The small peak to the right represents cells that bound the tested sample.
This product has been referenced in:
- Li Y et al. Single domain based bispecific antibody, Muc1-Bi-1, and its humanized form, Muc1-Bi-2, induce potent cancer cell killing in muc1 positive tumor cells. PLoS One 13:e0191024 (2018). Read more (PubMed: 29357376) »
- Woller SA et al. Inhibition of Neuroinflammation by AIBP: Spinal Effects upon Facilitated Pain States. Cell Rep 23:2667-2677 (2018). Flow Cyt ; Mouse . Read more (PubMed: 29847797) »