HRP Anti-6X His tag®抗体[4D11] (ab202004)
Key features and details
- HRP Mouse monoclonal [4D11] to 6X His tag®
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
HRP Anti-6X His tag®抗体[4D11]
参阅全部 6X His tag® 一抗 -
描述
HRP小鼠单克隆抗体[4D11] to 6X His tag® -
宿主
Mouse -
偶联物
HRP -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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表位
This antibody was shown to recognize an epitope (HHHHHHGS) encoded by the polylinker of several expression vectors (including pDS:RBSII, pQE-30, and pQE-50). -
阳性对照
- WB: E. coli Positive Control (Escherichia coli ) Whole Cell Lysate
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常规说明
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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纯度
IgG fraction -
克隆
单克隆 -
克隆编号
4D11 -
骨髓瘤
NS1 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab202004于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/5000.
|
说明 |
---|
WB
1/5000. |
靶标
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相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
图片
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HRP Anti-6X His tag® antibody [4D11] (ab202004) at 1/5000 dilution +
E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202004 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab202004 尚未被引用在任何文献中。