Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK 293 cells transfected with CACNB3-His)
Specification
HEK 293 cells transfected with CACNB3-His
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TX100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% goat serum, 0.1%TX100, 1XPBS
Secondary antibody
Name
Non-Abcam antibody was used: anti mouse Alexa Fluor 546
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Dilution
1/500
Additional data
Additional Notes
HEK 293 cells were transfected wirh CACNB3-His and stained with anti His Ab (red) 36 h after transfection. DAPI was used for nuclei staining (blue).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
提交于 Nov 20 2009