Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293 cells transfected with Beta3-His (CACNB3))
Loading amount
25 µg
Specification
HEK 293 cells transfected with Beta3-His (CACNB3)
Gel Running Conditions
Reduced Denaturing (10% SDS)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X PBS/Tween, 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: anti mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 55 kDa Non-specific: 85, 100, 130 kDa
Negative control
HEK-293 cells lysate
Additional data
Additional Notes
Line 1: HEK-293 lysate
Line 2: HEK-293 cells transfected with Beta3-His (CACNB3)
Line 2: HEK-293 cells transfected with Beta3-His (CACNB3)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
提交于 Nov 20 2009