重组Anti-6X His tag®抗体[3D5] - C-terminal (ab206504)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [3D5] to 6X His tag® - C-terminal
- Suitable for: ELISA, WB
- Reacts with: Species independent
概述
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产品名称
Anti-6X His tag®抗体[3D5] - C-terminal
参阅全部 6X His tag® 一抗 -
描述
大鼠单克隆抗体[3D5] to 6X His tag® - C-terminal -
宿主
Rat -
特异性
ab206504 reacts to recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins. It also has affinity for internal 6X His tag® but no affinity for 6X His tag® fused to amino terminus.
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经测试应用
适用于: ELISA, WBmore details -
种属反应性
与反应: Species independent -
免疫原
Recombinant fragment corresponding to 6X His tag®. Purified His tagged Fab fragment.
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常规说明
ab206504 is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
3D5 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab206504于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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说明 |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
靶标
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相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab206504 被引用在 2 文献中.
- Rocchi A et al. Autoantibodies to synapsin I sequestrate synapsin I and alter synaptic function. Cell Death Dis 10:864 (2019). PubMed: 31727880
- Lindner P et al. Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. Biotechniques 22:140-9 (1997). PubMed: 8994661