Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse pancreatic cancer primary cell lines)
Loading amount
25 µg
Specification
mouse pancreatic cancer primary cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/2000
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
8 minute(s) and 0 second(s)
Bands
Specific: ca. 120 kDa
Additional data
Additional Notes
Notch1 intracellular domain with His-tag over-expressed in mouse cells, in incrementally increasing amounts of the plasmid.
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提交于 Feb 09 2011