Application
Western blot
Sample
Human Purified protein (purified His-tagged lipase, SPINK1)
Loading amount
1 µg
Specification
purified His-tagged lipase, SPINK1
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Positive control
Several His-tagged proteins were loaded.
Additional data
Additional Notes
This antibody does not work at all for His-tagged proteins.
Abcam response
Thank you for your review. I am sorry you are experiencing trouble with this antibody. Although our antibodies are batch tested there is occasionally a problem with a particular vial. As your vial is not working as stated on the datasheet and is within the 120 day guarantee period we would like to offer you a replacement antibody or a credit to your account.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Miklos Sahin-Toth
Verified customer
提交于 Aug 12 2010