Application
Western blot
Sample
Human Purified protein (recombinant human fibronectin domain expressed in)
Loading amount
1 µg
Specification
recombinant human fibronectin domain expressed in
Gel Running Conditions
Reduced Denaturing (12% BisTris NuPAGE gel)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/3000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: tris-buffered saline + 0.1% Tween20
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL (SuperSignal WestDura)
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 25 kDa
Negative control
Uninduced (i.e., no IPTG induction) samples were included in lanes 2-4.
Additional data
Additional Notes
Lane 1: Benchmark His-tagged protein ladder (Invitrogen)
Lanes 2-4: protein from uninduced (no IPTG) cultures
Lanes 5-7: protein from induced (with IPTG) cultures 1000, 175, 50 ng in lanes 5, 6, and 7, respectively
Lanes 2-4: protein from uninduced (no IPTG) cultures
Lanes 5-7: protein from induced (with IPTG) cultures 1000, 175, 50 ng in lanes 5, 6, and 7, respectively
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提交于 Sep 09 2008