重组Anti-5 Lipoxygenase/5-LO抗体[EP6072(2)] - BSA and Azide free (ab227991)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP6072(2)] to 5 Lipoxygenase/5-LO - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-5 Lipoxygenase/5-LO抗体[EP6072(2)] - BSA and Azide free
参阅全部 5 Lipoxygenase/5-LO 一抗 -
描述
兔单克隆抗体[EP6072(2)] to 5 Lipoxygenase/5-LO - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Rat, Human
预测可用于: Dog不与反应: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Fetal thymus, K562 and HUVEC lysates. Human prostate and Human tonsil tissue. HUVEC cells.
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常规说明
ab227991 is the carrier-free version of ab169755.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP6072(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- APC Anti-5 Lipoxygenase / 5-LO antibody [EP6072(2)] (ab310831)
- PE Anti-5 Lipoxygenase / 5-LO antibody [EP6072(2)] (ab310907)
- Alexa Fluor® 594 Anti-5 Lipoxygenase / 5-LO antibody [EP6072(2)] (ab311696)
- Alexa Fluor® 568 Anti-5 Lipoxygenase / 5-LO antibody [EP6072(2)] (ab312971)
- Alexa Fluor® 555 Anti-5 Lipoxygenase / 5-LOantibody [EP6072(2)] (ab313180)
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Compatible Secondaries
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Conjugation kits
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227991于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 78 kDa.
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说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 78 kDa. |
靶标
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功能
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes. -
通路
Lipid metabolism; leukotriene A4 biosynthesis. -
序列相似性
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain. -
翻译后修饰
Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus. -
细胞定位
Cytoplasm. Nucleus matrix. Nucleus membrane. Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association. - Information by UniProt
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数据库链接
- Entrez Gene: 477753 Dog
- Entrez Gene: 240 Human
- Entrez Gene: 25290 Rat
- Omim: 152390 Human
- SwissProt: P09917 Human
- SwissProt: P12527 Rat
- Unigene: 89499 Human
- Unigene: 9662 Rat
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别名
- 5 Lipoxygenase antibody
- 5 LO antibody
- 5 LOX antibody
see all
图片
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ab169755 staining 5 Lipoxygenase/5-LO in HUVEC (human umbilical vein/vascular endothelium) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/130. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (black).
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169755).
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Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169755).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 5 Lipoxygenase/5-LO with ab169755, unpurified, at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169755).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This IHC data was generated using the same anti-5 Lipoxygenase/5-LO antibody clone, EP6072(2), in a different buffer formulation (ab169755).
ab169755 staining 5 Lipoxygenase/5-LO in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/100). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (1)
ab227991 被引用在 1 文献中.
- Li C et al. Qishen granules inhibit myocardial inflammation injury through regulating arachidonic acid metabolism. Sci Rep 6:36949 (2016). WB . PubMed: 27833128