Anti-4E-T抗体(ab6034)
Key features and details
- Goat polyclonal to 4E-T
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-4E-T抗体
参阅全部 4E-T 一抗 -
描述
山羊多克隆抗体to 4E-T -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse
-
免疫原
Synthetic peptide corresponding to Human 4E-T aa 972-985 (C terminal).
Sequence:AKVISVDELEYRQ
-
常规说明
GenBank Accession Number – NP_062817.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
Our Abpromise guarantee covers the use of ab6034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 140-150 kDa. A 1 hour primary incubation is recommended for this product. The predicted molecular weight is 108 kDa according to NP_062817, however our observation agrees with that of Dostie et al, PMID: 10856257. |
靶标
-
功能
Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism. -
组织特异性
Widely expressed. -
细胞定位
Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. - Information by UniProt
-
数据库链接
- Entrez Gene: 56478 Human
- Entrez Gene: 74203 Mouse
- Omim: 607445 Human
- SwissProt: Q9NRA8 Human
- SwissProt: Q9EST3 Mouse
- Unigene: 517559 Human
- Unigene: 255649 Mouse
-
别名
- 2610509L04Rik antibody
- 4E T antibody
- 4E-T antibody
see all
图片
-
Western blot - Anti-4E-T antibody (ab6034)
ab6034 at 0.25µg/ml staining approximately 140-150kDa band of 4E-T in HEK293 Lysate by Western blot (ECL). ab6034 at 0.25µg/ml staining approximately 140-150kDa band of EIF4ENIF1 in HEK293 Lysate by Western blot (ECL).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4E-T antibody (ab6034)ab6034 at 5ug/ml staining 4E-T in human pancreas tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
数据表及文件
文献 (3)
ab6034 被引用在 3 文献中.
- Kamenska A et al. The DDX6-4E-T interaction mediates translational repression and P-body assembly. Nucleic Acids Res 44:6318-34 (2016). PubMed: 27342281
- Waghray S et al. Xenopus CAF1 requires NOT1-mediated interaction with 4E-T to repress translation in vivo. RNA 21:1335-45 (2015). PubMed: 26015597
- Marnef A et al. RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor. Mol Biol Cell 23:213-24 (2012). ICC/IF ; Human . PubMed: 22090346
