Anti-4E-T抗体(ab6034)
Key features and details
- Goat polyclonal to 4E-T
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-4E-T抗体
参阅全部 4E-T 一抗 -
描述
山羊多克隆抗体to 4E-T -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human 4E-T aa 972-985 (C terminal).
Sequence:AKVISVDELEYRQ
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常规说明
GenBank Accession Number – NP_062817.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab6034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 140-150 kDa. A 1 hour primary incubation is recommended for this product. The predicted molecular weight is 108 kDa according to NP_062817, however our observation agrees with that of Dostie et al, PMID: 10856257. |
靶标
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功能
Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism. -
组织特异性
Widely expressed. -
细胞定位
Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner. - Information by UniProt
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数据库链接
- Entrez Gene: 56478 Human
- Entrez Gene: 74203 Mouse
- Omim: 607445 Human
- SwissProt: Q9NRA8 Human
- SwissProt: Q9EST3 Mouse
- Unigene: 517559 Human
- Unigene: 255649 Mouse
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别名
- 2610509L04Rik antibody
- 4E T antibody
- 4E-T antibody
see all
图片
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Western blot - Anti-4E-T antibody (ab6034)ab6034 at 0.25µg/ml staining approximately 140-150kDa band of 4E-T in HEK293 Lysate by Western blot (ECL). ab6034 at 0.25µg/ml staining approximately 140-150kDa band of EIF4ENIF1 in HEK293 Lysate by Western blot (ECL).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4E-T antibody (ab6034)ab6034 at 5ug/ml staining 4E-T in human pancreas tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
数据表及文件
文献 (3)
ab6034 被引用在 3 文献中.
- Kamenska A et al. The DDX6-4E-T interaction mediates translational repression and P-body assembly. Nucleic Acids Res 44:6318-34 (2016). PubMed: 27342281
- Waghray S et al. Xenopus CAF1 requires NOT1-mediated interaction with 4E-T to repress translation in vivo. RNA 21:1335-45 (2015). PubMed: 26015597
- Marnef A et al. RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor. Mol Biol Cell 23:213-24 (2012). ICC/IF ; Human . PubMed: 22090346
