常规说明Description: Control cell lysates from 293T human kidney cells were cultured in the presence of media containing 10% FCS. Cells were lysed in RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM NaF, 1 mM Na3O4, 1 mM PMSF, 1 µg/ml aprotinin, 1 µg/ml leupeptin, 1 µg/ml pepstatin, 1% NP-40, 0.25% sodium deoxycholate). 200 µg of cellular protein was mixed with the same volume of 2X SDS-PAGE loading buffer.
Growth Properties: adeherent Morphology: epithelial Organ: kidney Antigen Expression: SV40 T antigen Age: fetus
The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line.
形式Lyophilized:Simply reconstitute to desired concentration with sterile, distilled water or 1XPBS, adding glycerol to a final concentration of 10%. Add 100 µl distilled water containing 10% glycerol to the sample for a final concentration of 2 mg/ml lysate. Heat reconstituted cell/tissue lysate at 100C for 10min, cool down and load on to gel.
存放说明Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 0.788% Tris HCl, 1.54% DTT, 2% Sodium lauryl sulfate, 10% Glycerol, 0.1% Bromophenol blue
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裂解物说明The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab95494 被引用在 1 文献中.
- Gonzalez-Vicente A et al. FRUCTOSE REABSORPTION BY RAT PROXIMAL TUBULES: ROLE OF SODIUM-LINKED COTRANSPORTERS AND THE EFFECT OF DIETARY FRUCTOSE. Am J Physiol Renal Physiol N/A:N/A (2018). PubMed: 30565998