25(OH) Vitamin D ELISA试剂盒(ab213966)
Key features and details
- Sensitivity: 1.98 ng/ml
- Range: 0.5 ng/ml - 1010 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
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产品名称
25(OH) Vitamin D ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sample 1 20 225.8ng/ml 1.6% Sample 2 20 37.3ng/ml 2.1% Sample 3 20 5.3ng/ml 3.4% 批次间 样品 n Mean SD CV% Sample 1 30 297.2ng/ml 11.5% Sample 2 30 38.4ng/ml 15.8% -
样品类型
Serum, Plasma -
检测类型
Competitive -
灵敏度
1.98 ng/ml -
范围
0.5 ng/ml - 1010 ng/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 91.9 84% - 97.9% -
检测时间
1h 30m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
25(OH) Vitamin D ELISA kit is a complete kit for the quantitative determination of 25(OH) Vitamin D3 and 25(OH) Vitamin D2 and was tested in human plasma and serum samples.
It is recommended that you read the entire kit insert before proceeding with the assay.
Recent research efforts have shown that Vitamin D levels affect various disease states and are being linked with numerous indicators of well-being in humans. These include bone diseases such as osteoporosis and arthritis, but also additional disease including hypertension, diabetes, cancer and heart disease to name a few. Our Vitamin D ELISA kit offers an alternative to labor intensive and/or costly methods of testing for Vitamin D levels in human plasma and serum.
The transformation to the active form of Vitamin D begins with 7-dehydrocholesterol being acted upon by UV rays from the sun to form parent Vitamin D3. Alternatively, Vitamin D can be ingested as parent Vitamin D2 from various food sources, native or fortified. These parent compounds are transported to the liver and undergo hydroxylation to 25(OH) Vitamin D. This metabolite is then transported to the kidney where it undergoes a second hydroxylation to 1,25(OH)2 Vitamin D, the biologically active form of Vitamin D. It is important to note that levels of Vitamin D metabolites increase proportionately with increased uptake of parent Vitamin D. This combined with the greater half-life and stability of 25(OH) Vitamin D in circulation versus the active form (25 days versus 8 hours) are the reasons that the detection of the 25(OH) Vitamin D metabolite is used as the indicator for total Vitamin D concentration.
We have received feedback that this kit is not compatible with mouse samples.
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说明
Dissociation Buffer is added to wells coated with donkey anti-sheep IgG antibody. Standards and Samples are then added to these wells and dissociation of 25(OH) Vitamin D from Vitamin D Binding protein allowed to occur.
A solution of alkaline phosphatase conjugated 25(OH) Vitamin D3 is then added, followed by a solution of sheep monoclonal antibody to 25(OH) Vitamin D.
During incubation at room temperature the antibody binds the 25(OH) Vitamin D from the sample/standard or conjugate in a competitive manner and is itself captured by the anti-sheep IgG antibody. The plate is then washed, leaving a complex with bound 25(OH) Vitamin D from samples or the Alkaline phosphatase conjugate.
A pNpp substrate solution is added initiating an alkaline phosphatase catalyzed reaction that generates a yellow color in the solution.
Stop solution is added to stop the substrate reaction and the resulting yellow color is read at 405nm. The amount of signal is inversely proportional to the level of 25(OH) Vitamin D in the sample.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 25(OH) Vitamin D Antibody 1 x 5ml 25(OH) Vitamin D Conjugate (100X) 1 x 50µl 25(OH) Vitamin D Standard 1 (1010 ng/mL) 1 x 40µl 25(OH) Vitamin D Standard 2 (279 ng/mL) 1 x 40µl 25(OH) Vitamin D Standard 3 (71.6 ng/mL) 1 x 40µl 25(OH) Vitamin D Standard 4 (24.4 ng/mL) 1 x 40µl 25(OH) Vitamin D Standard 5 (4.8 ng/mL) 1 x 40µl 25(OH) Vitamin D Standard 6 (0.5 ng/mL) 1 x 40µl 25(OH) Vitamin D3 Conjugate Diluent 1 x 6ml Dissociation Buffer 1 x 10ml Donkey anti-sheep IgG Microplate (12 x 8 wells) 1 x 96 tests Plate Sealer 3 units pNpp Substrate 1 x 20ml Sample Diluent 1 x 1.8ml Stop Solution 1 x 5ml Wash Buffer 4 Concentrate (20X) 1 x 20ml -
研究领域
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab213966 被引用在 12 文献中.
- El Askary A et al. Effect of Application of Treadmill Training on Metabolic Control and Vitamin D Level in Saudi Patients with Type 2 Diabetes Mellitus. Comput Math Methods Med 2022:3059629 (2022). PubMed: 35140804
- Shafie A et al. Association of Vitamin D Deficiency and Vitamin D Receptor Genetic Variants With Coronary Artery Disease in Type 2 Diabetic Saudi Patients. In Vivo 36:1444-1452 (2022). PubMed: 35478145
- Youssry S et al. Association of hepatitis B vaccine response to vitamin D supplementation and ultraviolet B (UVB) exposure during different time intervals in experimental animals. Immunol Res 70:537-545 (2022). PubMed: 35585421
- Alqudah M et al. Correlation between vitamin D and serum brain derived neurotropic factor levels in type 2 diabetes mellitus patients. Biomed Rep 16:54 (2022). PubMed: 35620310
- Salih MM et al. Evaluation of CD4+:CD8+ Ratio in Patients With Cervical Cancer and the Levels of Inflammatory Markers. In Vivo 36:2414-2421 (2022). PubMed: 36099148