17 beta Estradiol ELISA试剂盒(ab108667)
Key features and details
- Sensitivity: 8.68 pg/ml
- Range: 20 pg/ml - 2000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Mammals
概述
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产品名称
17 beta Estradiol ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 20 <=9% 批次间 样品 n Mean SD CV% Overall 3 <=10% -
样品类型
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Competitive -
灵敏度
8.68 pg/ml -
范围
20 pg/ml - 2000 pg/ml -
回收率
101.09 %
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实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mammals -
产品概述
Abcam’s 17 beta Estradiol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 17 beta Estradiol in serum and plasma (citrate).
A 96-well plate has been precoated with anti-Estradiol IgG. Samples and the Estradiol-HRP conjugate are added to the wells, where Estradiol in the sample competes with the added Estradiol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol in the sample and the intensity is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Washing Solution 1 x 50ml 17 beta Estradiol Control 1 x 0.5ml 17 beta Estradiol Standard 0 1 x 0.5ml 17 beta Estradiol Standard 1 (20 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 2 (120 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 3 (300 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 4 (600 pg/mL) 1 x 0.5ml 17 beta Estradiol Standard 5 (2,000 pg/mL) 1 x 0.5ml 17 beta Estradiol-HRP Conjugate 1 x 22ml Anti-17 beta Estradiol IgG Coated Microplate (12 x 8 wells) 1 unit Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
研究领域
图片
数据表及文件
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SDS download
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Datasheet download
文献 (45)
ab108667 被引用在 45 文献中.
- Iijima H et al. Age-related matrix stiffening epigenetically regulates α-Klotho expression and compromises chondrocyte integrity. Nat Commun 14:18 (2023). PubMed: 36627269
- Wolf DC et al. Sex-specific differences in KCC2 localisation and inhibitory synaptic transmission in the rat hippocampus. Sci Rep 12:3186 (2022). PubMed: 35210456
- Zhong X et al. Sex specificity of pancreatic cancer cachexia phenotypes, mechanisms, and treatment in mice and humans: role of Activin. J Cachexia Sarcopenia Muscle 13:2146-2161 (2022). PubMed: 35510530
- Rodrigues J et al. Oestrogen enforces the integrity of blood vessels in the bone during pregnancy and menopause. Nat Cardiovasc Res 1:918-932 (2022). PubMed: 36531334
- Liu X et al. The AMPK-mTOR axis requires increased MALAT1 expression for promoting granulosa cell proliferation in endometriosis. Exp Ther Med 21:21 (2021). PubMed: 33235630